Problems and Solutions in Cloning and Expressing Eukaryotic Genes

Shows how a prokaryotic E. coli cell can be induced to replicate a eukaryotic human gene by using an expression vector. Demonstrates the process of making complementary DNA.

Figure 16.13, Purves's Life: The Science of Biology, 7th Edition; Figure 20.5, page 369, Campbell's Biology, 5th Edition

Genomic Libraries

Shows the “shotgun” approach to cloning a human gene, where no gene is targeted until it is time to select the bacterial colony. Shows how genes cut by restriction enzymes can be used with genomic libraries.

Figure 20.3, page 367, Campbell's Biology, 5th Edition; unknown source

Amplifying DNA: the Polymerase Chain Reaction (PCR)

Demonstrates the process of the polymerase chain reaction.

Unlabeled figure, page 371, Campbell's Biology, 5th Edition